Production of In-Vitro Fertilized Embryos and Result of Transfer with Fresh or Frozen Embryos for Hanwoo and Holstein Cattle
2005
Kim, Y.J. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: [email protected] | Kim, H.C. (Chonbuk National University, Jeonju, Republic of Korea) | Seo, S.H. (Jeonju-Zoo, Jeonju, Republic of Korea) | Jeong, K.N. (Livestock Breeding Station, Livestock Development and Research Institute of Chonrabuk-do, Gimje, Republic of Korea) | Kim, Y.S. (Livestock Breeding Station, Livestock Development and Research Institute of Chonrabuk-do, Gimje, Republic of Korea) | Lee, H.R. (Chonbuk National University, Jeonju, Republic of Korea) | Shin, D.S. (Livestock Breeding Station, Livestock Development and Research Institute of Chonrabuk-do, Gimje, Republic of Korea) | Jo, S.W. (Livestock Breeding Station, Livestock Development and Research Institute of Chonrabuk-do, Gimje, Republic of Korea) | Kim, S.H. (Chonbuk National University, Jeonju, Republic of Korea)
The ovaries from Hanwoo and Holstein were collected from labattoir and transferred to laboratory. Oocytes were aspirated and incubated in CO₂ incubator for 24 hours for maturation. Oocytes were coincubated with the sperms for 5 hours. Cleaved oocytes were selected 48 hours after coincubation and half of the medium was changed newly every 48 hour until blastocyst formation. Cleavage rate and blastocyst rate were investigated according to different breeds and different status of cumulus cells surrounding the oocytes. Blastocysts were either transferred to the recipients or frozen until use.
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