Studies on the In Vitro Development of Cloned Embryos by Somatic Cell Nuclear Transfer in Korean Native Goats
2005
Park, H.S. (Jinju National University, Jinju, Republic of Korea), E-mail: [email protected] | Kim, T.S. (Jinju National University, Jinju, Republic of Korea) | Jung, S.Y. (Jinju National University, Jinju, Republic of Korea) | Lee, Y.H. (Jinju National University, Jinju, Republic of Korea) | Jung, J.Y. (Jinju National University, Jinju, Republic of Korea)
The present study was conducted to examine some factors affecting in vitro development of oocytes from somatic cell nuclear transfer (SCNT) in Korean native goats. Recipient oocytes were surgically collected after superovulation by using CIDR and FSH, PMSG, hCG and estrous synchronization in Korean Native goats. For nuclear transfer, the fibroblasts from caprine ear cells and fetal fibroblasts were surgically harvested and were cultured in vitro until cell confluency in serum-starvation condition (TCM-199 + 0.5% FBS) for 3 to 5 days. The zona pellucidae of matured oocytes were partially drilled by laser irradiation.
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