Quantitative assay of glycosphingolipid and determination of binding affinity for Shigatoxin 2e in pig
Dong, B.Y.(Korea Green Cross Co., Yongin, Republic of Korea) | Kim, S.H.(University of Guelph, Gulph, Ontario, Canada) | Cho, H.H.(National Veterinary Research and Quarantine Service, Seoul, Republic of Korea) | Kim, Y.I.(National Veterinary Research and Quarantine Service, Busan, Republic of Korea) | Lee, W.W.(Pusan Institute of Health and Environment, Busan, Republic of Korea) | Kim, K.S.;Kang, H.J.;Kim, Y.H.(Gyeongsang National University, Jinju, Republic of Korea)E-mail:yho157@nongae.gsnu.ac.kr
This study was designed to quantitative assay of cellular receptor globotriosyl ceramide(Gb₃) and globotetraosyl ceramide(Gb₄) and to determine binding affinity of the receptors for Shigatoxin 2e in difference with day olds of pig. The amount of Gb₃ and Gb₄ extracted from each swine tissue were detected in order of spleen, ileocecum, kidney, and jejunum. The amount of Gb₃ and Gb₄ extracted from each swine ileocecum and jejunum in different day old swine were detected in order of 24, 6, 98, 12 and 35, but the difference of amount was not significant.
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