Production of Grapevine Rootstock "5BB" Plants as Influenced by Bioreactor Culture Method and pH and EC of Nutrient Solution
2004
Shim, S.W. (National Highland Agricultural Research Institute, RDA, Pyeongchang, Republic of Korea) | Hahn, E.J. (Chungbuk National University, Cheongju, Republic of Korea) | Paek, K.Y. (Chungbuk National University, Cheongju, Republic of Korea)
Shoot tips of grape rootstock '5BB' (selected at the Korean Fruit Research Institute) were cultured to obtain disease-free plantlets. The shoots were cut into single nodes (2 cm with a leaf), and cultured in bioreactors with three different culture methods;continuous immersion (with or without air exchanges) and temporary immersion (with air exchanges). Shoot growth was greatly affected by air exchanges in bioreactors, showing 1.5 times higher growth under air exchanges compared to without air exchanges.
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