Activation by Combined Treatment with Cycloheximide and Electrical Stimulation of In-Vitro Matured Porcine Improves Subsequent Parthenogenetic Development
2006
Naruse, Kenji (Chungnam National University, Daejeon, Republic of Korea) | Kim, .H.R. (Chungnam National University, Daejeon, Republic of Korea) | Shin, Y.M. (Chungnam National University, Daejeon, Republic of Korea) | Chang, S.M. (Chungnam National University, Daejeon, Republic of Korea) | Lee, H.R. (Chungnam National University, Daejeon, Republic of Korea) | Tarte, Vaishali (Chungnam National University, Daejeon, Republic of Korea) | Quan, Y.S. (Chungnam National University, Daejeon, Republic of Korea) | Kim, B.C. (Chungnam National University, Daejeon, Republic of Korea) | Park, T.Y. (Chungnam National University, Daejeon, Republic of Korea) | Choi, S.M. (Chungnam National University, Daejeon, Republic of Korea) | Park, C.S. (Chungnam National University, Daejeon, Republic of Korea) | Jin, D.I. (Chungnam National University, Daejeon, Republic of Korea), E-mail: dijin@cnu.ac.kr
Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treament. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide (10μg/ml) for 0, 5, 10, 20, 30 min abd then activated by electrical pulse treatment and cultured in PZM-3 for 8 days.
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