A case of sex determination by amplification of SRY and Amelogenin gene in horse
2005
Cho, G.J. (Korea Racing Association, Gwachon, Republic of Korea), E-mail: chogj@kra.co.kr | Lee, S.Y. (Korea Racing Association, Gwachon, Republic of Korea) | Yang, Y.J. (Korea Racing Association, Gwachon, Republic of Korea)
The objective of present study was to ascertain sex determination for individual identification, parentage control, and sex chromosome anomalies in horse. PCR amplification products of the equine sex determining region of the Y chromosome gene (SRY) and amelogenin gene (AMEL) were detected by using agarose gel electrophoresis. A normal sire and foal Ⅱ showed 1 SRY band (430 bp) and 3 AMEL (AMELX, AMELY, and AMELX/Y) band, 175 bp, 160 bp, 190 bp, respectively, and a normal dam and foal Ⅰ showed a single AMELX band (175 bp). These results enables a quick diagnosis for sex determination prior to cytogenetic analysis.
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