Nucleotide sequence analysis and expression of NSP4 gene of avian rotavirus
2005
Shin, I.H. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, W.Y. (Chungang University, Seoul, Republic of Korea) | Lee, S.C. (Chungbuk National University, Cheongju, Republic of Korea) | Kang, S.Y. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: sykang@chungbuk.ac.kr
The nonstructural protein 4 (NSP4) of rotavirus encoded by gene 10, plays an important role in rotavirus pathogenicity. In this study, NSP4 gene of avian rotavirus (AvRV-1, AvRV-2) was analyzed and expressed using baculovirus expression system. The sequence data indicated that the NSP4 gene of AvRV-1 and AvRV-2 were 727 bases in length, encoded one open reading frame of 169 amino acids beginning at base 41 and terminating at base 550, and had two glycosylation sites. Nucleotide sequences of NSP4 gene of AvRV-1 and AvRV-2 exhibited a high degree of homology (88.1±7.6%) with avian rotaviruses, namely Ty1, Ty3 and PO-13.
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