Identification of viruses and phytoplasma infecting delphinium (Delphinium L.) plants in Lithuania
Navalinskiene, M.(Institute of Botany, Vilnius (Lithuania))E-mail:msamuit@botanika.lt | Samuitiene, M.(Institute of Botany, Vilnius (Lithuania))E-mail:fitvirus@botanika.lt | Jomantiene, R.(Institute of Botany, Vilnius (Lithuania))
Plants of delphinium (Delphinium L.) exhibiting symptoms characteristic of viral and phytoplasmal diseases were collected at Botanical gardens and different floriculture farms in Lithuania. Viral diseases were expressed by symptoms of leaf distortion mottling, light green or yellow ringspotting. The causal agents of viral diseases, Cucumber mosaic cucumoviris (CMV) and Tomato ringspot nepovirus (ToRSV), were isolated and identified by the methods of test-plants and electron microscopy. Phytoplasmal symptoms of delphinium included general yellowing and stunting of plants, and virescence of flowers. Amplification of phytoplasmal 16S rRNA gene sequence, in polymerase chain reactions (PCRs) containing phytoplasma universal primer pair R16F2n/R2 and template DNA extracted from diseased plants, confirmed that the plants were infected by phytoplasma. The 1.2 kbp 16S rDNA prodcut was subjected to single enzyme digestions with 10 different restriction endonucleases. RFLP analysis revealed that the delphinium plants were infected by a phytoplasma belonging to group 16Srl (aster yellows phytoplasma group), subgroup B (1-B, aster yellows subgroup).
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