One-step Separation of 30K Protein from the Silkworm Hemolymph by Anion-exchange Chromatography and Its Effect on the Proliferation of Human Cells
2005
Shin, H.C. (Inha University, Incheon, Republic of Korea) | Joung, C.H. (Inha University, Incheon, Republic of Korea) | Choi, Y.S. (Inha University, Incheon, Republic of Korea) | Lim, S.M. (Inha University, Incheon, Republic of Korea) | Han, K.B. (Hanson Biotech Co., Ltd., Hannam University, Daejeon, Republic of Korea) | Koo, Y.M. (Inha University, Incheon, Republic of Korea) | Park, T.H. (Seoul National University, Seoul, Republic of Korea) | Kim, D.I. (Inha University, Incheon, Republic of Korea), E-mail: kimdi@inha.ac.kr
In order to investigate the feasibility of 30K protein from silkworm (Bombyx mori) hemolymph (SH) on the proliferation of human cells, a simple separating procedure by anion-exchange chromatography system with Q-Sepharose fast flow gel was established. The 30K protein was eluted with an optimized condition of 0.16 M sodium chloride in 20 mM tris buffer (pH 9.0). The separated 30K protein at three concentrations of 0.04, 0.12, and 0.4 mg/ml was added to the culture medium with various human cells, such as chondrocytes, periosteum-derived cells, and MRC-5 cells, and their growth rates were measured.
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