Isolation and Characterization of Exopolysaccharide Producing Lactic Acid Bacteria from Kimchi
2006
Kim, H.J. (Chosun University, Gwangju, Republic of Korea) | Chang, H.C. (Chosun University, Gwangju, Republic of Korea), E-mail: hcchang@mail.chosun.ac.kr
Three slime-forming lactic acid bacteria were isolated from Kimchi and shown to produce viscous exopolysaccharides (EPS) in sucrose media. The isolated strains, GJ2, C3 and C11, were identified as Leuconostoc kimchii, Leuconostoc citreum and Leuconostoc mesenteroides, respectively, by examining their metabolic characteristics and determining their 16S rDNA sequences, Leu. kimchii GJ2, Leu. citreum C3 and Leu. mesenteroides C11 exhibited high viability (maintained initial viable cell count of 10∨8 CFU/ml) in 0.05 M sodium phosphate buffer (pH 3.0) for 2 h, in artificial gastric juice for 2 h and in 0.3% oxgall for 24 h.
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