Expression of Soluble Recombinant Integrin Alpha V and Beta 3 from SF21 Cells with Baculovirus Expression Vector for Targeted Screening of Integrin Antagonists
2005
Kang, I.C. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected] | Min, B.S. (Chungbuk National University, Cheongju, Republic of Korea) | Ahn, E.H. (Chungbuk National University, Cheongju, Republic of Korea) | Son, H.S. (Seoul National University, Seoul, Republic of Korea) | Baek, K.H. (Pochon CHA University, CHA General Hospital, Seoul, Republic of Korea)
We examined production of membrane domain-truncated integrin α∧v and β₃ in an insect cell line, SF21, by using baculovirus expression vector for screening of new ligands interacted with integrin α∧vβ₃. Molecular cloning of the integrin receptors was carried out by truncation of transmembrane and cytoplasmic domains. Extracellular domains of integrin α∧v and β₃ were expressed in SF 21 cells and purified by His-tag affinity column chromatography with high purity. Activities of purified recombinant integrin α∧v and β₃ were confirmed by solid phase integrin binding assay via direct interaction method of integrin α∧vβ₃-vitronectin on protein microarray chip.
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