Molecular Characterization and Infectious cDNA Clone of a Korean Isolate of Pepper mild mottle virus from Pepper
2005
Yoon, J.Y. (Seoul Women's University, Seoul, Republic of Korea) | Hong, J.S. (Seoul Women's University, Seoul, Republic of Korea) | Kim, M.J. (Seoul Women's University, Seoul, Republic of Korea) | Ha, J.H. (Seoul Women's University, Seoul, Republic of Korea) | Choi, G.S. (National Horticultural Research Institute, RDA, Suwon, Republic of Korea) | Choi, J.K. (Kangwon National University, Chuncheon, Republic of Korea) | Ryu, K.H. (Seoul Women's University, Seoul, Republic of Korea), E-mail: ryu@swu.ac.kr
A Korean isolate of Pepper mild mottle virus (PMMoV-Kr) was isolated from a diseased hot pepper plant and its biological and molecular properties were compared to that of PMMoV-J and PMMoV-S. The genomic RNA of PMMoV-Kr consists of 6,356 nucleotides. The nucleotide and amino acid sequences identities of four viral proteins and two noncoding regions among PMMoV-Kr, PMMoV-S and PMMoV-J were 96.9% to 100.0% and 97.5% to 98.6%, respectively. Full-length cDNA amplicon of PMMoV-Kr was directly amplified by RT-PCR with a set of 5'-end primer anchoring T7 RNA promoter sequence and 3'-end virus-specific primer.
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