Production of Recombinant Trehalose Synthase from Thermus caldophilus GK24
2006
Choi, J.Y. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Cha, W.S. (Chosun University, Gwangju, Republic of Korea) | Shin, H.J. (Chosun University, Gwangju, Republic of Korea), E-mail: shinhj@chosun.ac.kr
A gene (GeneBank AF 135796) coding for a trehalose synthase from Thermus caldophilus GK24 was cloned into Escherichia coli K12 using five vector systems. The constitutive expression system (pHCETS) which shows the highest trehalose synthase activity from flask culture of recombinant E. coli was selected for the production of trehalose from maltose. For the shake flask culture, the final dry cell weight was 0.9 g/L and the trehalose synthase activity was 25 U/mL.
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