Effect of pH and phosphate supply on acid phosphatase activity in cereal roots

Sarapatka, B.,Palacky Univ., Olomouc . Faculty of Science, Dept. of Ecology and Environmental Sciences, Tr. Svobody 26, CZ-771 46 Olomouc; Dudova, L.; Krskova, M.

Effect of pH and phosphate supply on acid phosphatase activity in cereal roots

2004

Sarapatka, B.,Palacky Univ., Olomouc (Czech Republic). Faculty of Science, Dept. of Ecology and Environmental Sciences, Tr. Svobody 26, CZ-771 46 Olomouc | Dudova, L. | Krskova, M.

Enzymes in soils originate from animal, plant and microbial sources and the resulting soil biological activity includes the metabolic processes of all these organisms. The literature shows that under favourable conditions microorganisms supply most of the enzyme activity. The effect of plants on soil enzymatic activity is due to changes in organic matter content and microbial populations, but is also formed by accumulated enzymes and by continuously released extracellular and endocellular enzymes; all of which originate in the plant root. Our research studies acid phosphatase activity linked to the previous source, by which we mean cultivated plants. Evaluation was carried out on the root systems of both the chosen species and cereal varieties and also in nutrient medium on which crops were planted under conditions of changing pH and phosphorus supply. Different varieties of winter wheat, spelt, barley and rye were used and after sterilization the seeds were sown on Murashige/Skoog nutrient medium with pH 5.6, 6.2 and 6.8 and a phosphorus supply between 30-160 mg P2O5 L-1 of medium. After 10 days of cultivation the plant roots were harvested, homogenized and the acid phosphatase activity was measured. The results show that the acid phosphatase activity in the root system of various species and cereal cultivars is negatively correlated with increasing pH and available phosphorus level in the nutrient medium.

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