Mass culture development of marine algae genus Dunaliella, as the source of beta-carotene
1999
Udom Sittiphuprasert(Kasetsart University, Bangkok (Thailand). Kasetsart University Research and Development Institute. Sriracha Fisheries Research Station | Orapin BhumibamonKasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agro-Industry. Department of Biotechnology | Piyapong ChotipuntuKasetsart University, Bangkok (Thailand). Kasetsart University Research and Development Institute. Sriracha Fisheries Research Station | Phunthip WisespongpundKasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Fisheries. Department of Marine Science | Likit ChuchitKasetsart University, Bangkok (Thailand). Kasetsart University Research and Development Institute. Sriracha Fisheries Research Station)
Marine algae, Dunaliella salina var. DS 1197 from subproject 1 KIP 20.1.38 revealed the possibility to produce beta-carotene for the industry by culturing in John medium at 15-25 percent salt, pH 7.6+-0.02, 28-36 deg C, under controlled light condition of 20,000-35,000 luxes and light-dark period of 16 : 8 hours. The segregation of pure specimen is conducted by colonal culture method. The sample was pipetted for only one celi and cultured in liquid agar, transfered to test tube, to 50 ml Erlenmeyer flasks, for 14-21 and 10-15mday-periods respectively, ultil 4-5*10**(6) cell/ml was obtained. The specimen were further propagated in the 200 ml test tube, in 500 ml Erlenmeyer fasks under the controlled condition with light, temperature and carbondioxide supplied for 22 days at 40 percent innocculume size in order to get 28-30 litres of marine algae 2-3*10**(6) cell/ml. And then the marine algae were transferred to propagated in 20 litres plastic bags till the Dunaliella salina were reached. After that the marrine algae were transferred to propagate in out door mass cultured in 500 litres tank.
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