Evaluation of methods for sporidia production and germination of Ustilago maydis
2005
Mehraban, Fahmideh
During 2002-2003 many specimens of infected common smut were collected from Karaj , Varamin and shahriar and transferred to the laboratory . Teliospores were surface – disinfested in copper sulfate solution , spread on PDA and incubatedfor 5 days at 23 C.Sporidia from the resulting colonies were spread on agar , and colonies arising from single sporidia were Any isolated. ِ aboveground part of th e plant can become infected when airborn sporidia from germinated teliospores, or germinating teliospores Contact susceptible tissue. The opt. temp. for the germination of teliospores of Ustilago maydisin vitro was 28-30C. Germination was stimulated by sucrose but was not influenced by light. In comparative trials with varios inoculation methods , injection of sporidia were the most effective at all growth stages , while the teliospore Or sporidia whole inoculation method showed the highest infection.
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