Application of a soild-phase fluorescence immunoassay to determine ampicillin residues in muscle tissue of olive flounder (Paralichthys olivaceus)
2006
Jung, W.C. (Gyeongsang National University, Jinju, Republic of Korea) | Ha, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Chung, H.S. (Gyeongnam Provincial Government, Changwon, Republic of Korea) | Heo, S.H. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, S. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, H.J. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: hujang@gnu.ac.kr
Parallux∨TM, a solid-phase fluorescence immunoassay (SPFIA) developed for antibiotics residue detection in milk, was applied for analysis of fish muscle. The recommended therapeutic dose of ampicillin (100 mg/kg body weight, withdrawal period 7 days) was orally administered to a group of 25 olive flounders (Paralichthys olivaceus) for consecutive five days. Muscle was sampled after drug treatment 1st, 2nd, 3rd, 4th and 5th day. The concentration of ampicillin in muscle, determined by SPFIA, was compared with that of internal standard (10 ppb as ampicillin).
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