Analysis for nucleotide sequence of the membrane protein gene of porcine epidemic diarrhea virus Chinju99
2006
Baquilod, Greta Salvae V. (Provincial Veterinary Office, Borongan, Philippines) | Yeo, S.G. (Kyungpook National University, Daegu, Republic of Korea), E-mail: sgyeo@knu.ac.kr
Porcine epidemic diarrhea virus (PEDV) strain Chinju99, which was previously isolated from piglets suffering from severe diarrhea was used to characterize the membrane (M) protein gene to establish the molecular information, and the results will be useful in elucidating concepts related to molecular pathogenesis and antigenic structures of PEDV isolates. The Chinju99 M gene generated by reverse transcription and polymerase chain reaction (RT-PCR) consisted of 681 bases containing 22.3% adenine, 22.3% cytosine, 23.1% guanine and 32.3% thymine nucleotides, and the GC content was 45.4%.
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