Solubilization Conditions for Hydrophobic Membrane Protein, Oleosin, in Soybeans
2007
Kim, H. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, S.Y. (Chungbuk National University, Cheongju, Republic of Korea) | Han, N.S. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: namsoo@chungbuk.ac.kr | Tao, Bernard Y. (Purdue University, West Lafayette, IN, USA), E-mail: tao@purdue.edu
Oleosins are amphipathic proteins found in soybeans that give stability to oil-bodies by surrounding them. Furthermore, oleosins have broad biotechnological applications in protein purification and in immobilization as a tagging partner. In this study we provide the proper combinations of detergents and chaotropes for solubilization of oleosins. The solubilization effects of each detergent were compared by the results of SDS-PAGE analysis, from the density measurement of each band using an image analysis program.
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