Immunogenicity of the recombinant Pasteurella multocida toxin for development of subunit vaccine against swine atrophic rhinitis
2007
Lee, J.M. (Sungkyunkwan University, Suwon, Republic of Korea), E-mail: jlee@skku.edu
Pasteurella multocida D:4 producing Pasteurella multocida toxin (PMT) is a causal pathogen in atrophic rhinitis in pigs. To investigate the protective immunity and vaccination effect of recombinant PMT, the gene for PMT was isolated from the infective P. multocida D:4. The 2.3 kb XhoI/PstI fragment (PMT2.3) of PMT gene was expressed in E. coli BL21 (DE3) using the induced expression vector system. The recombinant protein of PMT2.3 having molecular weight of 84 kDa was purified by Ni-affinity column chromatography.
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