Development of catalase gene nuclear DNA-based marker for population genetic analysis in Thai teak (Tectona grandis L.f.)
2006
Jongkon Cheua-ngam(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Center for Agricultural Biotechnology) | Volkaert, Hugo(National Science and Technology Development Center (NSTDA-BIOTEC), Pathum Thani (Thailand)) E-mail:hugo.v@ku.ac.th
Teak Catalase gene was amplified and cloned using consensus PCR primers designed based on sequences from other plant species. The obtained teak Cat DNA sequences were used to develop specific primers. The specific primer set could successfully amplify a specific DNA fragment from teak in all populations studied. Using PCR-SSCP, 5 different alleles were detected. The tested nuclear gene primer set had considerable potential as a DNA marker for population analysis in teak. This approach could be used to specifically amplify fragments in other plant species and applied to study evolution, population genetics, outcrossing rate and mating patterns.
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