Analysis of genetic diversity in Cicer arietinum L using random amplified polymorphic DNA markers

Singh, R.; Prasad, C.D.; Singhal, V.; Randhawa, G.J., National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, New Delhi

Analysis of genetic diversity in Cicer arietinum L using random amplified polymorphic DNA markers

2002

Singh, R. | Prasad, C.D. | Singhal, V. | Randhawa, G.J., National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, New Delhi (India)

PCR-based random amplified polymorphic DNA (RAPD) markers were employed to assess genetic diversity in 23 chickpea genotypes. Forty of the 100 random primers screened revealed polymorphism among the genotypes. Most of the primers revealed single polymorphic band, and only 14.12 of the products were polymorphic. Estimates of genetic similarity based on Jaccard's coefficient ranged form 0.92 to 0.99, indicating narrow genetic variability among the genotypes based on RAPD markers. The 23 chickpea genotypes formed two major clusters in the dendrogram. The low RAPD polymorphism among chickpea genotypes suggests that more number of polymorphic primers need to be analysed to determine genetic relationships. It was observed that RAPD analysis employing 30 polymorphic primers could provide better estimates of genetic relationships in chickpea.

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