Optimum media for outdoor large-scale and indoor small-scale batch style culture of Nannochloropsis oculata
2008
Okauchi, M.(National Research Inst. of Aquaculture, Minami-Ise, Mie (Japan)) | Yamada, T. | Ozaki, A.
A new enriched seawater medium for Nannochloropsis (ESM-NA medium) using analytical grade inorganic salts for indoor small-scale culture of Nannochloropsis oculata was formulated by removing ineffective chemicals as nutrients from Guillard F medium. In addition, a fertilized seawater medium for outdoor large-scale culture of the alga (FSM-NA medium) was devised in consideration of cost reduction. The composition of ESM-NA medium was NaNO3; 4.41 mM, NaH2PO4.H2O; 90microM, Fe-EDTA; 29.7microM, MnCl2.4H2O; 2.2microM, ZnSO4.7H2O; 0.38microM and CoCl2.6H2O; 0.126microM, while that of FSM-NA medium was KNO3 ; 5.3 mM, NH4H2PO4; 0.66 mM, Clewat 32; 20 mg/l and Clewat Fe; 3 mg/l. The growth rate of N. oculata in FSM-NA medium was higher than that in YASHIMA medium which has been commonly used in Japanese hatcheries. Moreover, it can be considered that the contamination of FSM-NA medium into larval rearing water at the same time as the supplementation of N. oculata was little effect to the survival and the growth of fish larvae. Therefore, FSM-NA and ESM-NA media seem to be optimal for outdoor large-scale and indoor small-scale culture of N. oculata, respectively.
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