Genetic transformation of a high molecular weight glutenin (Glu-1Dx5) to rice [Oryza sativa] cv. Fatmawati
2009
Wada, Y.(Utsunomiya Univ. (Japan)) | Carsono, N. | Anas | Tong, L. | Yoshida, T.
In order to improve rice dough functionality, we co-transformed the Glu-1Dx5 gene encoding a high molecular weight (HMW) glutenin subunit Dx5 from bread wheat, Triticum aestivum L. and either bar gene conferring resistance to herbicide bialaphos or hpt gene conferring resistance to hygromycin B to rice callus cells of cv. Fatmawati. We molecularly characterized 9 plants regenerated from bialaphos-containing medium and 63 plants from hygromycin-containing medium. The Glu-1Dx5 gene was detected by PCR analysis in 15 transgenic Tsub(0) Plants. Further analysis of Tsub(1) and Tsub(2) plants revealed that some transgenic plants carried the Glu-1Dx5 gene. Analysis of the endosperm extracts of Tsub(2) plants by SDS-PAGE revealed the existence of a protein similar in size to the wheat Glu-1Dx5 gene product, suggesting successful expression of the transgene. These plants will be incorporated into breeding program for further assessment of their benefits.
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