Molecular Cloning, Sequencing, and Biochemical Characterization of Streptomyces rimosus TDP-glucose 4,6-dehydratase
2007
Kim, S.S. (Myongji University, Yongin, Republic of Korea) | Suh, J.W. (Myongji University, Yongin, Republic of Korea) | Kim, J.Y. (Jeju Biodiversity Research Institute (JBRI), Jeju, Republic of Korea) | Hyun, C.G. (Jeju Biodiversity Research Institute (JBRI), Jeju, Republic of Korea), E-mail: [email protected]
TDP-(Thymidine diphosphate)-glucose 4,6-dehydratase (EC 4.2.1.46), which converts TDP-glucose to TDP-4-keto-6-deoxyglucose, is a common essential enzyme in the biosynthesis of all types of 6-deoxyhexose. Genes that encode TDP-glucose 4,6-dehydratase are found within gene clusters that contain biosynthetic genes for natural products containing 6-deoxyhexose moieties. The genes that are required for the bioxynthesis of secondary metabolites, lipopolysaccharide, and O-antigen are also usually clustered in the genome in competent organisms [Kim et al., 2000]. In our previous work, we therefore designed oligonucleotide primers to amplify DNA fragments of the genes for actinomycetes TDP-glucose synthase, which could then be used to efficiently amplify and isolate the genes for sugar moiety biosynthesis in actinomycetes [Hyun et al., 2000].
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