Occurrence of Seven Honeybee Viruses and Research of Disease Occurrence in Korean Apiaries
2008
Choi, Y.S. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea), E-mail: [email protected] | Lee, M.L. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Lee, M.Y. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) | Lee, K.G. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea)
Mortality of honeybees is a serious problem that beekeepers have to face periodically in Korea and worldwide. The presence of RNA viruses, in addition to other pathogens may be one of its possible causes. In this work, we detected Deformed wing virus (DWV), Israle Acute Paralysis Virus (IAPV), Black queen cell virus (BQCV), Cloudy wing virus (CWV), Kashmir bee virus (KBV), Sacbrood virus (SBV), Chronic bee paralysis virus (CBPV) in samples of korea honeybees with or without Varroa destructor and Nosema apis. The detection of viruses in all provinces, simultaneous co-infection of colonies by several viruses and the fact that 96.3% of the samples were infected with one or more virus, indicates they are widely spread in the region. Using uniplex and multiplex RT-PCR we screened honey bee colonies for the presence of several bee viruses, including DWV, IAPV, BQCV, KBV, CWV, and described the detection of mixed virus infections in bees from these colonies. Also, average spore number of Nosema apis was confirmed by 30,029 spores per 1 worker bee, and 128,750 spores were confirmed in Anseong that is 1,200, maximum place of infection in Gangneung that is lowest occurrence area. Average infection rate of chalk brood was 27.03%, and Cheorwon that is lowest infection area is 2.3% and Suncheon that is maximum infection area was 71.9%. Conclusively, investigated disease of the bee, and confirmed new virus that lead to bee disease, this is thought by valuable thing as data for development of beekeeping industry such as CCD (Colony Collapse Disorder)'s cause searching examination.
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