Characterization of EMS induced leaf senescing mutants in Arabidopsis

Raziuddin . Dept. of Plant Breeding and Genetics; Wollaston, V.B. . Dept. of Biological Sciences; Farhatullah . PARC Technology Transfer Inst.; Ali, S. . Dept. of Plant Breeding and Genetics

Characterization of EMS induced leaf senescing mutants in Arabidopsis

2007

Raziuddin (NWFP Agricultural Univ., Peshawar (Pakistan). Dept. of Plant Breeding and Genetics) | Wollaston, V.B. (London Univ., England (UK). Dept. of Biological Sciences) | Farhatullah (Agriculture Research Inst., Tandojam (Pakistan). PARC Technology Transfer Inst.) | Ali, S. (Faculty of Agriculture, Rawalakot (Pakistan). Dept. of Plant Breeding and Genetics)

Leaf senescence is a genetically programmed developmental process, mediated by biochemical changes. It is induced by developmental signals or environmental factors and the content of leaf are mobilized to developing leaves, flowers, fruits and the death of leaf tissue results. To investigate the genes involved in leaf senescence, both molecular and mutational approaches could be used. In the present experiment, mutagenic approach was used to understand the leaf senescence in Arabidopsis thaliana Landsberg erecta. Arabidopsis seeds were treated with a chemical mutagen, ethyl methane sulphonate (EMS). The mutagenized population was screened to identify delayed senescence mutants. Two different classes of Arabidopsis mutants were identified. Morphologically delayed senescing mutants; mds, were identified by robust structure, prolonged greenness, continuous flowering and increased number of leaves, branches and siliques. The biochemically delayed senescing mutants; bds, showed reduced protein degradation during senescence. Both these characters (mds and bds) were found heritable. The segregation of phenotypes in M3 suggested that both these characters are controlled by single dominant gene.

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