Tissue culture of oil palm (Elaeis guineensis) | การเพาะเลี้ยงเนื้อเยื่อปาล์มน้ำมัน
2009
Chayanit Distabanjong(Department of Agriculture, Bangkok (Thailand). Office of Biotechnology Research and Development) | Karsedis Distabanjong(Department of Agriculture, Bangkok (Thailand). Office of Biotechnology Research and Development) | Poomarin Wanichananan(Department of Agriculture, Bangkok (Thailand). Office of Biotechnology Research and Development) | Onrat Wongsri(Department of Agriculture, Bangkok (Thailand). Suratthani Oil Palm Research Center) | Arunee Jaithung(Department of Agriculture, Bangkok (Thailand). Suratthani Oil Palm Research Center)
Tissue culture of oil palm (tenera type),Surat-thani 3 had been done using immature embryo, immature inflorescence and young leaf primordia surrounding the cabbage as explants. Explants had been cultured on MS and Y3 media supplemented with various concentrations of dicamba for callus induction. Immature embryo showed the highest callus percentage at 83.30 percent on MS media supplemented with 10 microM dicamba. Immature influrescense and young leaf primordia showed the highest callus percentage at 15.8 percent on Y3 media supplemented with 15 microM dicamba, and 24.63 percent on MS media supplemented with 15 microM dicamba, respectively. Callus proliferation ability of immature embryo, immature inflorescence and young leaf primordia were 7.02 x , 3.87x ,and 9.13x, on MS media supplemented with 1 microM, 3 microM and 1 microM of dicamba, respectively. The percentage of embryogenic callus induction from the original callus were 50.01 percent 20.04 percent and 46.76 percent from immature embryo, immature inflorescence and young leaf primordia respectively on Y3 media supplemented with NAA 10 microM and abscisic acid 2 microM In addition, the development of somatic embryo from embryogenic callus of each explant parts were 40.08 percent, 13.36 percent, and 33.34 percent, respectively on the same media of embryogenic callus induction.
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