Mixtures of antagonistic strains to enhance control efficacy of bacterial leaf blight and growth promotion of rice | การผสมเชื้อปฏิปักษ์เพื่อเพิ่มประสิทธิภาพการควบคุมโรคขอบใบแห้ง และส่งเสริมการเจริญเติบโตข้าว
2009
Waraporn Pupakdeepan(Kasetsart University, Bangkok (Thailand). Faculty of Agriculture. Department of Plant Pathology) | Sutruedee Prathuangwong(Kasetsart University, Bangkok (Thailand). Faculty of Agriculture. Department of Plant Pathology)
New antagonistic strain D10 from rice rhizosphere in Angthong growing area and the effective strains, Bacillus amyloliquefaciens KPS46, Pseudomonas fluorescens SP007s, Serratia marcescens Spt360, and Paenibacillus sp. SW01/4 were mixed in different combination set and evaluated for increased efficacy in suppressing Xanthomonas oryzae pv. oryzae (Xoo) caused bacterial leaf blight of rice. Their potentials on increased indole-3-acetic acid (IAA) to enhanced plant growth of seeds treated with those antagonists were also characterized. A set of 3-strain combination of SP007s+Spt360+D10 was significantly greater in pathogen suppression with 11.6 mm inhibition diameter than other strain set of 2, 4, or 5. Interestingly, mixture technique of 3-strain co-culture in same NB flask for 48 h provided better inhibition of pathogen than immediate mixture of each 48 h old culture. We also found that mixed 3-strain co-culture increased highest IAA accumulation in resistant (Suphanburi1) and susceptible cultivars (Jusmine105) of rice with 0.13 and 0.07 micro g/ml respectively. The IAA obtained from leaves of seed treated with 3-stain mixture is a signal molecule that can trigger plant gene activation. The data obtained result in formula development of effective mixture for field application in the future.
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Эту запись предоставил Kasetsart University