Effect of GH and Octreotide on SOCS expressions in diet induced obese mice | 生长激素与奥曲肽对肥胖小鼠SOCS转录表达的影响

Китайский. 探讨在肥胖状态下，生长激素和奥曲肽对信号转导抑制因子（SOCS）转录表达的影响，为进一步探索生长激素通路参与肥胖形成的可能机制提供理论和实验依据。利用高脂日粮诱导小鼠肥胖模型，用外源性生长激素和生长抑素类似物奥曲肽处理肥胖小鼠，记录体质量变化，观察皮下脂肪、附睾脂肪和肾周脂肪的沉积情况，检测血清中甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白（HDL-C）和低密度脂蛋白（LDL-C）含量，同时利用RT-PCR法分析与生长激素密切相关的信号转导抑制因子1，2，3（SOCS1，SOCS2和SOCS3）mRNA的表达。外源性生长激素处理并不能减少肥胖小鼠脂肪沉积，反而增加了体脂含量（P＜0.01），血清中TG、TC、HDL-C和LDL-C含量无显著变化，而SOCS1、SOCS2和SOCS3 mRNA水平显著增加（P＜0.01）；奥曲肽处理减少了体脂含量（P＜0.01），血清中TG、TC和HDLC含量显著减少（分别为P＜0.05，P＜0.05 和P＜0.01），SOCS1和SOCS3 mRNA表达水平显著减少（P＜0.01），但SOCS2却显著增加（P＜0.01）。在肥胖状态下，生长激素并不能减少体脂沉积，但生长抑素类似物奥曲肽却能够减少体脂含量；SOCS1和SOCS3可能参与此作用机制，而SOCS2对于生长激素信号通路变化更敏感。

Английский. This study aimed at the effect of Growth Hormone and Octreotide on the transcription of Suppressor of cytokine signaling in order to provide experimental and theoretical evidence for the potential role of Growth Hormone signal pass-involved in regulating obesity synthesis. This study established high-fat-diet induced obese mice which are administrated with recombinant human Growth Hormone (rhGH) and Somatostatin Analogue Octreotide. The body weight, subcutaneous fat tissue, periepididymal fat pads and perirenal fat pads were weighed, total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured, and the expression of suppressor of cytokine signaling SOCS1, SOCS2 and SOCS3 which were related to GH were measured by RT-PCR. Determination of rhGH can't reduce but increase fat mass of obese mice (P＜0.01), no change was found about the contents of TG, TC, HDL-C, and LDL-C in serum, and the expression of SOCS1, SOCS2 and SOCS3 mRNA remarkably increased. After administration with Octreotide, body fat mass decreased significantly (P＜0.01), the contents of TG,TC and HDL-C in serum decreased (P＜0.05, P＜0.05 and P＜0.01 respectively), the expression of SOCS1, SOCS3 mRNA reduced (P＜0.01) while SOCS2 mRNA increased (P＜0.01). GH could not reduce fat mass in obesity circumstance, but Octreotide could. So it is suggested that SOSC1, SOCS3 might be involved in this functional system, while SOCS2 has more close relationship with Growth hormone signal pathway.