Cell Growth and Lipid Production from Fed-batch Cultivation of Chlorella minutissima according to Culture Conditions
2009
Oh, S.H., Kangwon National University, Chuncheon, Republic of Korea | Han, J.G., Kangwon National University, Chuncheon, Republic of Korea | Kim, N.Y., Kangwon National University, Chuncheon, Republic of Korea | Cho, J.S., DooSan EcoBizNet, Chuncheon, Republic of Korea | Yim, T.B., DooSan EcoBizNet, Chuncheon, Republic of Korea | Lee, S.Y., Kangwon National University, Chuncheon, Republic of Korea | Lee, H.Y., Kangwon National University, Chuncheon, Republic of Korea
The culture condition of growing Chlorella minutissima was optimized to produce biodiesel for fed-batch cultivation. First, under heterotrophic cultivation, the optimum level of glucose was determined to be 10 g/L for 20 days. After, three cultivation conditions were operated: autotrophic, heterotrophic, and mixotrophic growth. The lipid level and the maximum cell concentration from the fed-batch heterotrophic process were 32.0 (%, v/v) and 15.0 (g-dry wt./L) in 20 L flask, respectively. In addition, since the relatively constant specific lipid production rate was observed as 0.040 (% lipid/g-dry wt./day) at the latter period of cultivation time, the fed-batch process could maintain continuous lipid production. Fed-batch process is higher than those values from the batch process. The lipids from the fed-batch process contained over 38% of C∧18, known as the suitable composition for the biodiesel application. For mixotrophic and heterotrophic growth under fed-batch condition, glucose was proved to be an appropriate carbon source for a large scale outdoor cultivation. For fed-batch cultivation, the feeding rate of seawater medium containing glucose was decided to be 0.5 L/day. The mixotrophic cultivation maintained maximum cell concentration of 24 (g-dry wt./L) and the lipid level of 43 (%, w/w). The lipid composition from this process was also proved to be suitable for the biodiesel production. The fatty acids from the mixotrophic growth contains 18% of C∧17 and 49% of C∧18, implying It also tells that Chlorella minutissima is a suitable resource of biodiesel. Especially, the mixotrophic cultivation with fed-batch process might be useful for the large scale cultivation for the biodiesel production.
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