Nutritional, Chemical and Physical Factors Affecting Somatic Embryo Formation and Germination in Pimpinella brachycarpa
2009
Na, H.Y., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Chun, C.H., Seoul National University, Seoul, Republic of Korea
Formation and germination of somatic embryo (SE) are affected by the concentration of plant growth regulators; MS medium strength, sucrose concentration, and the photosynthetic photon flux (PPF) level. The MS medium strength and sucrose concentration for an optimal condition of the SE formation and germination of Pimpinella brachycarpa were x0.5, sucrose 30 g L-¹ and x0.25, sucrose 30 g L-¹, respectively. When 0.05 mg L-¹ of kinetin was treated, the percentage of normal somatic embryo (NSE) formation was the greatest and the percentage of hyperhydric somatic embryo (HSE) formation was the lowest. SE germination rate was greater in treatment of IAA 0.05 mg L-¹ or BA 0.01 mg L-¹ than those of other treatments. Moreover, better SE formation and germination were found at dimmer lighting (20 μmol m-² s-¹ of PPF) than that of higher PPFs. SE formation and germination were also retarded at higher PPFs. Optimum PPF for SE formation and germination was 20 μmol m-² s-¹. After forming SE from EC 0.1 g for 4 weeks, EC 0.1 g resulted in 700 total SE formation. Culturing four SEs for four weeks resulted in a secondary SE formation, germination and eventually 52 plantlets. These results indicate that a huge proliferation rate was achieved at up to 8 weeks of EC at liquid medium suspension culture and SE at solid medium culture. Through the experiment, after inducing EC from EC, the mass of EC was increased by 25 times (from 0.05 to 1.30 g) when each of the nutritional and chemical factors was controlled at the optimized condition.
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