Isolation and Characterization of Flavanone 3-Hydroxylase Genes from a Korean Raspberry
2009
Lee, S.S., Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea | Lee, E.M., Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea | An, B.C., Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea | Cho, J.Y., Chonbuk National University, Jeonju, Republic of Korea | Kim, T.H., Daegu Haany University, Daegu, Republic of Korea | Choi, Y.O., Dongbu HiTek Co., Ltd., Daejeon, Republic of Korea | Chung, B.Y., Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea
Flavanone 3-hydroxylase (F3H) is one of the key enzymes acting during a bifurcation of the anthocyanin and flavonols branches. Here, we report on the isolation and characterization of an F3H gene from a Korean raspberry (Rubus coreanus Miquel). By rapid amplification of cDNA ends (RACE)-PCR, the full-length cDNA of the RcoF3H gene contains a 1,098-bp open reading frame (ORF) encoding a 365 amino acid protein with a calculated molecular weight of about 41.1 kDa and an isoelectric point (pI) value of 5.45. The comparison of the deduced amino acid sequence of RcoF3H with other F3Hs indicated that the protein is highly homologous with various plant species. The RcoF3H gene exists as a single-copy in R. coreanus and had three exons and two introns. Transcription analysis revealed that RcoF3H was expressed at similar levels in the flower, stem and leaf. In addition, the gene had a different expression pattern during fruit development. At the early stage of fruit development, the expression level was extremely low, but the levels dramatically increased during the progress of ripening. Our results suggest a correlation between anthocyanin accumulation and expression of the RcoF3H gene during the ripening of fruits.
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