Status of Aspergillus niger strains for pectinase production potential

Hannan, A. . Inst. of Mycology and Plant Pathology; Bajwa, R. . Inst. of Mycology and Plant Pathology; Latif, Z. . Inst. of Mycology and Plant Pathology

Status of Aspergillus niger strains for pectinase production potential

2009

Hannan, A. (Punjab Univ., Lahore (Pakistan). Inst. of Mycology and Plant Pathology) | Bajwa, R. (Punjab Univ., Lahore (Pakistan). Inst. of Mycology and Plant Pathology) | Latif, Z. (Punjab Univ., Lahore (Pakistan). Inst. of Mycology and Plant Pathology)

A multistep evaluation of 52 strains of Aspergillus niger was performed on the basis of polygalacturonase production. Plate method was employed for preliminary screening of isolates. The strains exhibiting relative clear zone with diameter above 1.386 were selected for final screening. Out of 52 strains, 23 were selected for further screening by using optimized enzyme assay method. The enzyme production by 23 selected strains was studied in submerged fermentation with pectin as the only carbon source. Maximum activity was observed in two isolates of A. niger (H 12 and H51) showing more than 0.700 units after 96 hrs incubation period. The four isolates (H06, H13, H45, and H46) gave the maximum activities after incubation for 72h whereas decline was observed at 96h.

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