Phylogenetic analysis of the 16S rDNA of a strain isolated from diseased larva of Anoplophora glabripennis (Motsch.)
2008
Deng Caiping | Liu Hongxia | Yan Xizhong | Wu Xuxia | Luo Youqing
[Objective]Study on the phylogenetic analysis of the 16S rDNA of strain BH-1 isolated from diseased larva of Anoplophora glabripennis(Motsch.) and its insecticidal characteristics. [Method] The strain was identified by routine method and inoculated on healthy Anoplophora glabripennis(Motsch.) for observing insecticidal effect, further 16S DNA was amplified by the specific primers for sequencing and homology analysis. [Result] The mortality of second instar of Anoplophora glabripennis(Motsch.) reached 72.7% 8d after 1010 cfu/ml BH-1 was inoculated. The homology of 16S DNA sequences between BH-1 and Serratia marcescens accessed in GenBank was 99.5%. Combined with the results of routine identification, BH-1 was identified as S. marcescens. [Conclusion]BH-1 could be used for biological control of Anoplophora glabripennis (Motsch.).
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