Induction on gynogenesis of Silurus astus using cold shock
2008
Cheng Guo | Li Xuejun | Qiao Zhigang | Shi Ling | Peng Xinliang
[Objective]The cold shock method was used to induce diploid gynogenesis of Silurus astus.[Mthod]The Silurus astus sperms were irradiated by ultraviolet then conducted fertilization. Different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus.[Result]Under such inducing condition that ultraviolet irradiated 15 min, cold shock started 5 min after fertilization and cold shock duration was 40 min, the survival rate of diploid Silurus astus reached the highest(8.5%).[Conclusion]The experiment laid foundation for culturing new excellent Silurus astus varieties and accumulated original files for further study of gynogenesis development mechanism
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