In vitro quality of cryopreserved rooster sperm from "Gallina Valenciana de Chulilla" breed. Preliminary results | Calidad in vitro del semen crioconservado de gallos de la raza Gallina Valenciana de Chulilla. Resultados preliminares
2011
Blanch, E., Centro de Investigación y Tecnología Animal, Castellón (España) | Tomás, C., Centro de Investigación y Tecnología Animal, Castellón (España) | Sansano, S. | Gómez, E.A., Centro de Investigación y Tecnología Animal, Castellón (España) | Casares, L. | Giménez, I. | Mocé, E., Centro de Investigación y Tecnología Animal, Castellón (España)
The "Gallina Valenciana de Chulilla" is an endangered autochthonous chicken breed from the Comunidad Valenciana which is currently being preserved. The objective of this study was to compare the in vitro quality (sperm motility and plasma membrane integrity) of sperm cryopreserved with two different protocols (each of them used a different cryoprotectant: glycerol or dimethylacetamide-DMA). Samples cryopreserved with glycerol exhibited higher in vitro sperm quality after cryopreservation than samples cryopreserved with DMA (67.5+/-1.7% vs. 25.4+/-1.7% total motile sperm and 80.7+/-1.8% vs. 39.9+/-1.8% sperm with intact plasma membrane). In conclusion, the protocol where glycerol was used preserved better the in vitro sperm quality after thawing than the DMA protocol.
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