Detection of Honeybee Virus from Bumblebee (Bombus terrestris L. and Bombus ignitus)
2010
Choi, Y.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Lee, M.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Hong, I.P., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Kim, N.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Kim, H.K., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Byeon, K.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Yoon, H.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea
Mortality of honeybees is a serious problem that beekeepers have to face periodically in Korea and worldwide. The presence of RNA viruses, in addition to other pathogens may be one of its possible causes. A survey on the occurrence of seven honeybee-pathogenic viruses was carried out using RT-PCR assays. In this work, we detected Deformed Wing Virus (DWV), Israle Acute Paralysis Virus (IAPV), Black Queen Cell Virus (BQCV), Cloudy Wing Virus (CWV), Kashmir Bee Virus (KBV), Sacbrood Virus (SBV), Chronic Bee Paralysis Virus (CBPV) in samples of Bumblebee (Bombus terrestris L. and Bombus ignitus). The detection of viruses in all Bumblebee samples, simultaneous co-infection of colonies by several viruses, indicates they are widely spread in the colony. Honeybee virus is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult and larval stage bees. Here, we report for the first time the occurrence of multi-virus infected bumble bees (B. terrestris and B. ignitus). For the present study, the detection of bee virus from the adult bumble bee, death adult bumblebee, mail bumble bee, pupa stage bumblebee and pollen to the infection cycle was investigated in the same colony. Using specific RT-PCR protocols for the detection of virus followed by sequencing of the PCR products we could demonstrate that the bumblebees were indeed infected with several bee viruses.
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