Polymorphisms of the poly(C)-tract of porcine encephalomyocarditis virus (EMCV) isolated in Korea
2010
Hyun, B.H., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kim, H.J., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kim, I.J., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Pyo, H.M., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kim, S.M., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kim, S.H., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Lim, S.I., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kim, J.J., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Song, J.Y., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea
Encephalomyocarditis virus (EMCV) belongs to the genus Cardiovirus within the family Picornaviridae. EMCV has been recognized either as a cause of mortality in young pigs, due to acute myocarditis, or of reproductive failure in sows. An EMCV K3 strain was isolated from the heart and brain in a mummified and aborted swine fetus in 1989. For the molecular characterization of the poly(C)-tract of EMCV Korean isolates, K3 strain, viral RNA was extracted and digested with RNase T1, and analyzed the length of the poly(C)-tract by polyacrylamide gel electrophoresis. The poly(C) regions also were amplified by RT-PCR and sequenced. The present study shows that K3 strain of EMCV had a short polymorphic poly(C) tracts (5 to 30 C's) with sequences consisting of C∧9, C∧10, C∧13, C∧14, C∧16, C∧20, CUC∧11, C∧8UCUC₃UC∧10, C∧9UCUC₃UC∧10, C∧10UCUC₃UC∧10, etc. These polymorphism of poly(C)-tracts of EMCV K3 strain implies the historical information of in vivo and/or in vitro passage.
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