Influence of thawing temperature on sperm survivability and fertility in buffaloes with frozen semen of Murrah bulls.

Malik, R.K.; Tuli, R.K.; Dipanker,; Singh, Pardeep

Influence of thawing temperature on sperm survivability and fertility in buffaloes with frozen semen of Murrah bulls.

2011

Malik, R.K. | Tuli, R.K. | Dipanker, | Singh, Pardeep

The effect of two thawing temperatures (37°Cfor30 sec. and 60°Cfor 7 sec.)on sperm survivability and conception rate in buffaloes with frozen semen of Murrah bulls was compared. Thawing at 60°C resulted in statistically significant increase (P0.05) in sperm survivability (motility,live spermatozoa and intact acrosomes) compared with thawing at 37°c. In all, 161 buffaloes were inseminated with frozen semen thawed at 37°Cand 159buffaloeswith semen thawed at 60°Cbetween 2006-07 and 2009-10. Conception rate of 64.15% and 50.31% was obtained with semen thawed at 60°C and 37°C, respectively, and the difference was significant (P0.05). Artificial insemination of buffaloes with frozen semen thawed at 60°C for 7 sec. could be safely used to improve both sperm survivability and conception rate in buffaloes.

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