Molecular Detection of Pasteurella multocida by Species and Capsular Type B Specific PCR-assay.

Varshney, Mayur; Taku, Anil Kumar; Dutta, Tapan Kumar; Chhabra, Rajesh

Molecular Detection of Pasteurella multocida by Species and Capsular Type B Specific PCR-assay.

2009

Varshney, Mayur | Taku, Anil Kumar | Dutta, Tapan Kumar | Chhabra, Rajesh

A total of 253 samples from cattle (52), buffalo (51), sheep (50), goat (50) and chicken (50) were screened for the presence of Pasteurella multocida. Out of these, 17 samples were found positive by species specific PCR (PM-PCR) with ~460 bp amplified product. Overall prevalence of P. multocida was found to be 6.7%. Out of 17 PM-PCR positive samples, 12 (70%) were found positive by capsular type-B specific (HSB-PCR) and multiplex PCR assays with amplification of ~620 bp product in both the cases. A total of 5 isolates of P. multocida were obtained in 17 PM-PCR positive samples of which 4 were of serotype B:2 and 1 was of serotype A:3. The PM-PCR and HSB-PCR assays can be performed directly on clinical samples from animals with reproducible results without any non-specific amplification. Multiplex PCR further reduces the time for the species and type specific detection of P. multocida.

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