In vitro multiplication of strawberry cv. Chandler

Negi, Meenaxi; Singh, C.P.; Srivastava, R.K.

In vitro multiplication of strawberry cv. Chandler

2008

Negi, Meenaxi | Singh, C.P. | Srivastava, R.K.

The investigation was conducted during the year 2005-2007 to standardize a protocol for production of strawberry plants. The establishment MS medium supplemented with BAP (0.5 mg/l) and kinetin (0.5 mg/l) was found best for culture survival (88.92%), earlier days to shoot emergence (9.81 days), shoot elongation (11.19 days) and number of shoots per explant (5.73). Different combinations of growth regulators BAP, IBA, kinetin, and GA3 were tried for further shoot multiplication. In BAP, IBA and GA3 combination MS medium supplemented with BAP (0.5 mg/l) in supplemented with IBA (0.5 mg/l) and GA3 (1.0 mg/l) resulted maximum shoot length (10.50 cm) while, maximum number of shoots (14.50) per explant was found on MS medium supplemented with BAP (0.5 mg/l) and IBA (0.5 mg/l). In BAP, kinetin and GA3 combination MS medium supplemented with BAP (0.5 mg/l), kinetin (0.5 mg/l) and GA3 (2.0 mg/l) was resulted maximum shoot length (4.60 cm), number of shoots (4.44), number of leaves (10.3) and number of fragments (3.71) per explant

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