Somatic embryogenesis and plant regeneration in cucumber.
Lou H. | Kako S.
The embryogenic capacity of seven cucumber (Cucumis sativus L.) cultivars were examined by tissue culture of cotyledon, young first-leaf, and internode explants. Somatic embryogenesis frequencies differed significantly among the tested cultivars, and 'Fushinarimidori' produced the highest number of embryos from either cotyledons of young first leaves. Cotyledon- and first-leaf-derived calluses produced more embryos than calluses from internodes. Somatic embryos were induced from 'Aonaga F1, internodes with relatively high sucrose levels (6% and 9%) in the initiation medium, the frequency of embryogenic callus formation from 'Fushinarimidori' cotyledon explants was > 90%. The highest yield of somatic embryos occurred in cultures initiated with high sucrose levels (9% or 12%), although 12% sucrose inhibited callus formation and growth. Somatic embryos germinated in a basal liquid medium supplemented with 0.5% activated charcoal, and they developed into well-shaped, healthy plantlets on semisolid medium with 1% sucrose.
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