The embryogenic capacity of seven cucumber (Cucumis sativus L.) cultivars were examined by tissue culture of cotyledon, young first-leaf, and internode explants. Somatic embryogenesis frequencies differed significantly among the tested cultivars, and 'Fushinarimidori' produced the highest number of embryos from either cotyledons of young first leaves. Cotyledon- and first-leaf-derived calluses produced more embryos than calluses from internodes. Somatic embryos were induced from 'Aonaga F1, internodes with relatively high sucrose levels (6% and 9%) in the initiation medium, the frequency of embryogenic callus formation from 'Fushinarimidori' cotyledon explants was > 90%. The highest yield of somatic embryos occurred in cultures initiated with high sucrose levels (9% or 12%), although 12% sucrose inhibited callus formation and growth. Somatic embryos germinated in a basal liquid medium supplemented with 0.5% activated charcoal, and they developed into well-shaped, healthy plantlets on semisolid medium with 1% sucrose.
显示更多 [+] 显示较少 [-]
AGRIS - 国际农业科技情报系统
Share
页码
v.906-909(8)
其它主题
Embryon vegetal; Cotyledon; Variete; Micropropagacion; Carbon vegetal; Regeneracion in vitro; Carbon activado; Regeneration in vitro
语言
英语
注释
references. AVAILABILITY: US (DNAL SB1.H6).
类型
Journal Article; Journal Part
来源
HortScience-:-a-publication-of-the-American-Society-for-Horticultural-Science (USA). (Aug 1994). v. 29(8) p. 906-909.
团体作者
Yamaguchi University, Yamaguchi, Japan.
Ministry of Agriculture, Newe Yaar (Israel). Agricultural Research Organization, Newe Yaar, Dept. of Vegetables.
2012-11-15
AGRIS AP