Phon khong kan soem ethinyl estradiol, methionine, lae lysine nai ahan kai khai thi mi malet nun to samatthaphap kan phalit khai.

In experiment 1, EE (ethinyl estradiol) was used at the levels of 0.4, 1.6 and 6.4 mg% of the rations, and laying hens alternately fed with the kapok seed and basal ration for 5 periods of 28 days each. The results indicated that EE supplement in the basal ration had no effect on egg production, egg-weight, hematology and egg quality as compared to control. However, EE supplement in the kapok seed ration did not reduce the detrimental effect of cyclopropenoid fatty acid (CPFA) in kapok seed in respect to egg production. EE supplement slowed down the decreasing rate of egg production during the periods in which the hens were fed with the "kapok seed ration" and normal performance was regained within a shortened period of time as compared to those hens receiving the "kapok seed ration" without EE supplement. In experiment 2, EE was used as a supplement to the kapok seed ration at the level of 1.6 and 6.4 mg in addition to methionine and lysine supplement at 0.25% each. The laying hens received the above ration in 4 consecutive periods of 28 days each. During these period, egg production significantly decreased (P < 0.05) while the hematological parameters increased. Nevertheless, egg quality was not affected. The addition of methionine and lysine in the kapok seed ration slightly reduced the toxic effect of CPFA, was not statistically significant. Histological study of the ovaries of the laying hens at the end of the experiment showed that there was no effect EE supplement on the number of follicles in the ovary. The ovary of the laying hens fed with the basal ration with EE supplement contained the same comparable number of folicles. However, there was a slight increased of the number of follicles of the ovary of hens fed with kapok seed ration either with or without EE supplement. A proliferation of kupffer's cells, broad sinusoids and hepatocyte degeneration in the liver of hens fed with kapok seed ration were observed. EE supplementation resulted in similar sinusoids and larger hepatocytes without proliferation of Kupffer's cells. Thus, the supplement did not affect the performance, hematological profile and egg quality of the layers. EE supplement in the kapok seed ration slightly decreased the harmful affects of CPFA.