Colonization of gram-negative bacterial community in aerobic hemp retting.
1988
Lim C.R. | Chung G.H. | Han H.E.
Dynamics of bacterial communities and its colonization under aerobic hemp retting were observed in air lift fermentor as a closed system, unlike conventional hemp retting as an open system. Dried hemp which was harvested in both 1986 and 1987 was retted at room temperature. Predominant community was facultatively anaerobic Gram-negative rods, and its density was increased from 3.0 x 10/7 cells/ml to 9.0 x 10/8 cells/ml. The density of facultatively anaerobic Gram-positive rods was maintained at the level of 5.0 x 10/6 cells/ml, and this Gram-positive bacterial community was not participating in retting. In the Gram-negative bacterial community during retting, five types of colonies were developed at early stage of pH 7.0-8.0, and thereafter, only three types were colonized till later stage, which were identified as pectolytic strain Erwinia salicis, Erwinia tracheiphila and Enterobacter agglomerans. A community of facultatively Gram-negative rods was mainly proliferated in stems and dispersed into liquor after 6-8 hours. Retting was terminated within 70-80 hours.
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