Virus thonyao khot thipen sahet rok baidang khong oi.
1985
Supaporn Nateewatana
Sugarcane mosaic virus (SCMV) was the causal agent of a common mosaic disease of sugarcane (Saccharum officinarum L.). Host range of this virus was limiteid to family Gramineae. Sorghum and sweet sorghum (Sorghum bicolor (L.) Moench) were highly susceptible. The virus was transmitted both by mechanical method and by aphids. The aphids, Hysteroneura setariae Thos and Rhopalosiphum maidis Fitch. had 6.67 and 3.33 percent transmission rates, respectively, in a stylet borne manner. The virus was not transmitted by Melanaphis sacchari Zehnt and through seeds of sorghum. The virus particle was a flexuous rod of 776 nanometer. Tubular, circular, semicircular and pinwheel inclusion bodies were found in infecnted sugarcane cells. Purification of this virus was difficult due to aggregation and precipitation of the virus particles. Opalescent band was not found following sucrose density gradient centrifugation but the virus detected at about 2.4 cm. from the miniscus by fractionator. Microprecipitin and decorate method in this study produced good results. Agar-gel double diffusion test with SLS and PVP -immunodifusion techniques showed no reaction.
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