Characterization of Light and Nitrogen Regulated Gene Expression Pathways in Marine Diatoms
2007
Smith, G. J.
To delineate pathways for the modulation of gene expression by marine diatoms in response to environmental cues. To elucidate the extent of light- and nitrogen-dependent transcriptional and translational control on the expression of nitrate reductase (NR), glutamine synthetase (GS) and the fucoxanthin- chlorophyll a/c pigment protein (FCP). The products of these genes determine the nitrogen assimilation and light harvesting capacity of marine diatoms and play a central role in the coupling of carbon and nitrogen metabolism in these cells. To characterize the genomic organization and promoter structure of NR, GS, and FCP for eventual identification of light- and nitrogen-responsive promoter elements.
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