Liquid and Gas Chromatographic Determination of 1,3,5-Trinitrobenzene in Water
1986
Rosencrance, Alan B. | Brueggemann, Ernst E.
Investigations conducted at the U.S. Army Medical Bioengineering Research and Development Laboratory (Aquatic Toxicology Section) have shown that 1,3,5-trinitrobenzene (TNB) is toxic to freshwater aquatic organisms. Further investigations are now being performed by the Aquatic Toxicology Section to determine the relative effects of TNB in constant and fluctuating applications on the water flea Daphia magna. In support of the Aquatic Toxicology Section, this report pertains to a task to develop chromatographic methods for the determination of TNB in water. The relative water solubility and available UV chromophore of TNB make it a suitable candidate for direct injection reversed phase high performance liquid chromatography (RP-HPLC). Furthermore, TNB's chemical and physical properties also make it an attractive choice for gas chromatography (GC) employing a nitrogen phosphorus detector (NPD). The liquid and gas chromatographic methods reported herein are intended to be quick, sensitive, and reproducible for the determination of TNB in water.
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