Developing a Zebrafish Model of NF1 for Structure-Function Analysis and Identification of Modifier Genes

Epstein, Jonathan A.

Developing a Zebrafish Model of NF1 for Structure-Function Analysis and Identification of Modifier Genes

2008

Epstein, Jonathan A.

This progress report summarizes the first year of activity of this project focused on the identification and characterization of the zebrafish orthologs of the neurofibromatosis type 1 genes. This project involves work within the Epstein laboratory and collaboration with the laboratory of Dr. Thomas Look at the Dana Farber Cancer Institute as a sub-contract. This progress report summarized the collaborative work including results from both groups. During the first year, significant progress has been made in the isolation and characterization of two zebrafish orthologs, znf1a and znf1b. The genes have been sequenced and their expression patterns identified. The knockdown phenotypes have been characterized and include overgrowth of glia and cardiovascular abnormalities. Work towards identification of stable mutant fish lines has begun. Manuscripts are in preparation.

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